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A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications
 

A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications

Article DOI: 10.3791/61822-v • 10:10 min • January 23rd, 2021
January 23rd, 2021 •
*1,2, *1,2, 1,2, 1,2, 1,2
1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, 2Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University
* These authors contributed equally

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Resumo
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This technique allows for the fast and simple preparation of whole-seed-sized resin section for the observation and analysis of cells, starch granules, and protein bodies in different regions of the seed.

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Keywords: Dry Sectioning Whole-seed-sized Resin Section LR White Resin Cereal Mature Kernels Cell Morphology Starch Granules Protein Bodies Sample Preparation Fixation Dehydration Infiltration Embedding Light Microscopy Quantitative Analysis
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