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Environment

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JoVE Journal Environment
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
 

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Article DOI: 10.3791/61825-v • 08:54 min • November 5th, 2020
November 5th, 2020 •
*1, *1, *1, *1
1Columbia Environmental Research Center, U.S. Geological Survey
* These authors contributed equally

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Environmental DNA assays require rigorous design, testing, optimization and validation before the collection of field data can begin. Here, we present a protocol to take users through each step of designing a species-specific, probe-based qPCR assay for the detection and quantification of a target species DNA from environmental samples.

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Keywords: Environmental DNA EDNA Quantitative PCR QPCR Species-specific Assay Assay Design Sequence Alignment NCBI Geneious MUSCLE Primer Design Probe Design Contamination Conservation Wildlife Management
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