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Moleculaire modulatie door lentivirus-geleverde specifieke shRNA's in endoplasmatisch reticulum gestreste neuronen
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Capítulos
- 00:05Introduction
- 00:50Primary Rat Cortical Neuron Cultures
- 02:25Lentivirus Production
- 04:41Lentivirus Infection
- 06:23Primary Neuron Cultures Stressed with Ganglioside GM2 Accumulation and Immunocytochemistry
- 07:43Neurite Atrophy Analysis
- 08:38Results: GM2 Accumulation-Induced Neuritic Atrophy Modulated by CN-Aα and CHOP Knock-Down in an Opposite Manner
- 10:07Conclusion
In de huidige studie wordt de expressie neergehaald van twee stroomafwaartse signaalcomponenten van de PERK-route, de cytoprotectieve calcineurine en de pro-apoptotische CHOP, door specifieke shRNA's te gebruiken. Op tegenovergestelde manieren moduleren deze de gevoeligheid van primaire corticale neuronen voor neurietatrofie na inductie van endoplasmatische reticulumstress.
Tags
Keywords:
Molecular ModulationLentivirusShRNAEndoplasmic Reticulum StressNeuronsNeural PathwaysNeurodegenerative DiseasesGM2 GangliosidosisCell CultureTrypsin EDTAHank’s Balanced Salt SolutionMechanical DissociationCell PlatingSerum-free MediumOligonucleotide AnnealingLentiviral VectorRestriction Enzyme DigestionLigationE. Coli Transformation
