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Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
 

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Article DOI: 10.3791/62221-v • 09:48 min • July 26th, 2022
July 26th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,3
1Department of Orthopedics, The First affiliated hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People's Hospital, 3Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health

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Here, we present a protocol to produce a large number of GMP-grade exosomes from synovial fluid mesenchymal stem cells using a 3D bioreactor.

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Keywords: Synovial Fluid Mesenchymal Stem Cells Exosomes 3D Bioreactor GMP-grade Cell Culture Flow Cytometry Microcarriers Cell Preparation Centrifugation Cell Density Cell Passage Cell Pellet Cell Suspension Fluorescent Antibody Cell Washing Cell Detection
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