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In vitro Analyse van E3 Ubiquitine Ligase Functie
 
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In vitro Analyse van E3 Ubiquitine Ligase Functie

Article DOI: 10.3791/62393-v • 06:06 min • May 14th, 2021
May 14th, 2021 •
1, 1,2, 1, 1,2
1Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 2Center for Molecular Medicine Cologne, University of Cologne

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De huidige studie biedt gedetailleerde in vitro ubiquitylatie assay protocollen voor de analyse van E3 ubiquitine ligase katalytische activiteit. Recombinante eiwitten werden tot expressie gebracht met behulp van prokaryote systemen zoals Escherichia coli-cultuur.

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Biologie Nummer 171
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