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Journal / Immunology and Infection / ヒト胚性幹細胞からの肝細胞様細胞誘導の効率的な方法…
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Immunology and Infection

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JoVE Journal Immunology and Infection
ヒト胚性幹細胞からの肝細胞様細胞誘導の効率的な方法
 
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ヒト胚性幹細胞からの肝細胞様細胞誘導の効率的な方法

Article DOI: 10.3791/62654-v • 08:05 min • May 6th, 2021
May 6th, 2021 •
*1,2,3, *1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College
* These authors contributed equally

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本稿では、ヒト胚性幹細胞(hESC)を、hESC分化中にアクチビンAおよびCHIR99021を確実なエンドドーム(DE)に継続的に補い、機能性肝細胞様細胞(HLC)に分化するための詳細なプロトコルを記述する。

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免疫学と感染症、問題171、肝細胞様細胞、ヒト胚性幹細胞、細胞分化、決定的内胚、アクチビンA、CHIR99021
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