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JoVE Journal Bioengineering
Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes
 

Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes

Article DOI: 10.3791/62889-v • 08:27 min • August 13th, 2021
August 13th, 2021 •
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1Grup d’Enginyeria de Materials (Gemat), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL)

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Here, a simple protocol is presented for producing mRNA nanoparticles based on poly(beta aminoester) polymers, easy to be tailored by changing the encapsulated mRNA. The workflow for synthesizing the polymers, the nanoparticles, and their in vitro essential characterization are also described. A proof-of-concept regarding immunization is also added.

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Keywords: MRNA Nanoparticles Poly(beta Aminoesters) Vaccination Nucleic Acids Polymeric Nanoparticles Lyophilization Polyplexes Transfection Hela Cells Fluorescence Microscopy
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