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Neuroscience

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JoVE Journal Neuroscience
Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
 

Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices

Article DOI: 10.3791/62959-v • 10:48 min • September 7th, 2021
September 7th, 2021 •
1,2, 1,2, 1,2, 1,2, 3,4, 3,4, 5, 3,4, 1,2, 1,2, 5, 1,2,6, 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Center for Brain & Disease Research, Laboratory of Neurobiology, , 3VIB Center for Brain & Disease Research, Research Group Molecular Neurobiology, , 4VIB Bio Imaging Core, KU Leuven - University of Leuven, 5Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 6Department of Neurology, University Hospitals Leuven

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We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.

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Generation Human Motor Units Functional Neuromuscular Junctions Microfluidic Devices Research Motor Neurons Health Disease Stem Cell Technology Reproducibility Neuromuscular Diseases Humanized Model Amyotrophic Lateral Sclerosis Motor Unit Sterilization Poly-L-ornithine PLO Solution
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