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JoVE Journal Neuroscience
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
 

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Article DOI: 10.3791/63471-v • 09:24 min • December 23rd, 2021
December 23rd, 2021 •
1,2, 1,2,3, 1,2,3, 1,2,3
1Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, 2UCL Queen Square Motor Neuron Disease Centre, University College London, 3UK Dementia Research Institute, University College London

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Using transgenic fluorescent mice, detailed protocols are described to assess in vivo axonal transport of signaling endosomes and mitochondria within motor and sensory axons of the intact sciatic nerve in live animals.

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In Vivo Imaging Axonal Transport Signaling Endosomes Mitochondria Live Anesthetized Mice Basal Physiology Of Axons Peripheral Neurodegeneration Mouse Model Of Disease Potential Treatments Pharmacological Agents Gene Therapies Motor And Sensory Neurons Organelles Imaging Motor Disease Models Aging Glass Micropipette Intramuscular Injections Sterile Surgical Drape Operating Microscope Surgical Tools And Instruments Anesthetized Mouse
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