Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Dissection and Immunostaining of Larval Salivary…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes
 

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

Article DOI: 10.3791/62989-v • 06:12 min • September 30th, 2021
September 30th, 2021 •
1,2, 1, 1, 3, 1
1Department of Cell Biology, The Johns Hopkins University School of Medicine, 2Tufts School of Medicine, 3Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

Capítulos

Resumo
Automatic Translation

The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.

Tags

Keywords: Salivary Glands Anopheles Gambiae Mosquito Larvae Dissection Immunostaining Disease Transmission Malarial Parasites Rab 11 Alexa Fluor 488 Nile Red Hoechst
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter