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Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
 

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Article DOI: 10.3791/63009-v • 04:35 min • October 7th, 2021
October 7th, 2021 •
1, 2,3, 4, 3, 1, 1,2,3,4
1Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, 2International Research Center for Medical Sciences (IRCMS), Kumamoto University, 3Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, 4X-Earth Center, Faculty of Advanced Science and Technology, Kumamoto University

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Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

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Keywords: Computed Tomography 3D Visualization Murine Hind-limb Vessels Sample Preparation Heparin Vasodilation Buffer Paraformaldehyde Contrast Medium Perfusion Hind-limb Ischemia Model Femoral Artery Femoral Vein Collateral Vessels
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