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Immunology and Infection

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JoVE Journal Immunology and Infection
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
 

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

Capítulos

Resumo
Automatic Translation

The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.

Tags

Keywords: Microinjection Transgenics Knockouts Strongyloides Parasites Worms Baermann Fecal-charcoal Nematode Growth Medium E. Coli HB101
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