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Developmental Biology

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JoVE Journal Developmental Biology
Murine Neural Plate Targeting door In Utero Nano-Injection (NEPTUNE) op Embryonale Dag 7.5
 
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Murine Neural Plate Targeting door In Utero Nano-Injection (NEPTUNE) op Embryonale Dag 7.5

Article DOI: 10.3791/63148-v • 10:49 min • February 14th, 2022
February 14th, 2022 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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In dit protocol beschrijven we hoe de vruchtwaterholte van de muis op E7.5 kan worden geïnjecteerd met lentivirus, wat leidt tot uniforme transductie van de gehele neurale plaat, met minimale schadelijke effecten op overleving of embryonale ontwikkeling.

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Ontwikkelingsbiologie Nummer 180
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