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JoVE Journal Developmental Biology
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
 

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Article DOI: 10.3791/63166-v • 06:12 min • February 10th, 2022
February 10th, 2022 •
1,2, 1
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 2DBT-AIST International Laboratory for Advanced Biomedicine, National Institute of Advanced Industrial Science and Technology

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Resumo
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A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.

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Keywords: Stab Wound Injury Optic Tectum Zebrafish Medaka Regenerative Capacity Brain Injury RGC Proliferation Differentiation Needle-mediated Injury Central Nervous System Regenerative Therapy PBS Perfusion Paraformaldehyde Fixation
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