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Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
 

Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae

Article DOI: 10.3791/63259-v • 09:43 min • November 22nd, 2021
November 22nd, 2021 •
1, 1, 1, 1, 1,2, 1,2
1Centre for Discovery Brain Sciences, University of Edinburgh Medical School: Biomedical Sciences, 2Center for Regenerative Therapies at the TU Dresden

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The present protocol describes a method to induce tissue-specific and highly reproducible injuries in zebrafish larvae using a laser lesion system combined with an automated microfluidic platform for larvae handling.

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Keywords: Laser-induced Injuries Spinal Cord Regeneration Zebrafish Larvae Semi-automated Technique VAST Equipment Anesthetizing Larvae Automated Zebrafish Imaging Laser Ablation Python IDE Script Management Plate Handling Microscope Focusing Reproducibility
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