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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
 

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

Article DOI: 10.3791/63528-v • 06:59 min • June 22nd, 2022
June 22nd, 2022 •
1,3, 3, 2,4,5, 1,3,5
1Molecular Medicine, Hospital for Sick Children, 2Cell Biology, Hospital for Sick Children, 3Department of Physiology, University of Toronto, 4Department of Paediatrics, University of Toronto, 5Department of Biochemistry, University of Toronto

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This protocol describes a method for the study of electrogenic membrane proteins by measuring changes in membrane potential. This assay provides a platform for the functional readout of multiple ion channels endogenously expressed in epithelial cell lines.

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Fluorescence-based Assay Membrane Potential High-throughput Functional Study Endogenous Ion Channels Epithelial Cell Lines Modulators Exogenous Chemical Probe Small Molecule Modulators Cystic Fibrosis Calu-3 Cells Caco-2 Cells T75 Flask EMEM Medium Fetal Bovine Serum Penicillin Streptomycin Phosphate-buffered Saline Trypsin EDTA
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