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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
 

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

Article DOI: 10.3791/63742-v • 05:33 min • July 26th, 2022
July 26th, 2022 •
1, 2
1Electron Microscopy Laboratory, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Science and Technology

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The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.

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Keywords: Light-induced In Situ Transmission Electron Microscopy Liquid-soft Matter Interaction Bacteria Photosensitizer Sample Preparation Liquid Cell TEM Electron Dose Laser Light Intensity
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