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Preparation of Human Myocardial Tissue for Long-Term Cultivation
 

Preparation of Human Myocardial Tissue for Long-Term Cultivation

Article DOI: 10.3791/63964-v • 10:58 min • June 2nd, 2022
June 2nd, 2022 •
1,2, 1,2, 1,2,3, 4,5, 1,6, 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich

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We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.

Tags

Human Myocardial Tissue Long-term Cultivation Ex Vivo Biomimetic Chamber Pre And Afterloads Physiological Environment Tissue Contraction Stimulation Protocols Vital Contraction Parameters Refractory Period Therapeutic And Cardiotoxic Drug Effects Cardiovascular Medicine Cultivation Chambers Graphite Electrodes Circuit Board Petri Dish Lid Slicing Buffer
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