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Forberedelse af humant myokardievæv til langvarig dyrkning
 
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Forberedelse af humant myokardievæv til langvarig dyrkning

Article DOI: 10.3791/63964-v • 10:58 min • June 2nd, 2022
June 2nd, 2022 •
1,2, 1,2, 1,2,3, 4,5, 1,6, 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich

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Vi præsenterer en protokol for ex vivo dyrkning af humant ventrikulært myokardievæv. Det giver mulighed for detaljeret analyse af sammentrækningskraft og kinetik samt anvendelse af præ- og efterbelastning for at efterligne det in vivo fysiologiske miljø nærmere.

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Medicin udgave 184 myokardievæv human dyrkning ex vivo øget gennemstrømning sammentrækning
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