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Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
 

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Article DOI: 10.3791/63999-v • 09:00 min • June 10th, 2022
June 10th, 2022 •
1, 2, 3, 1
1Department of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, 2Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, 3University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute, University of Delaware

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This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

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Keywords: Vascular Endothelial Cells Adipose Tissue Vascular Beds Vascular Cell Biology Vascular Dysfunction Tissue Dissection Adipose Depot Isolation HEPES Buffer Peritoneal Cavity
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