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Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices
 

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

Article DOI: 10.3791/64013-v • 04:08 min • July 22nd, 2022
July 22nd, 2022 •
1, 2, 3, *3, *2
1Department of Integrative Biology, Oklahoma State University, 2Department of Physiology and Biophysics, University of Colorado Anschutz, 3Advanced Light Microscopy Core, University of Colorado Anschutz
* These authors contributed equally

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Visualizing myelination is an important goal for many researchers studying the nervous system. CARS is a technique that is compatible with immunofluorescence that can natively image lipids within tissue such as the brain illuminating specialized structures such as myelin.

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Keywords: CARS Coherent Anti-Stokes Raman Spectroscopy Myelination Brain Tissue Myelin Lipids CH2 Bonds Microscopy Fluorescence Imaging Immunohistochemistry Multiple Sclerosis Aging Autism Laser Alignment Forward CARS Epi CARS Confocal Imaging
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