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Kombination af 3D magnetisk kraftaktuator og multifunktionel fluorescensbilleddannelse for at studere kernemekanobiologi
 
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Kombination af 3D magnetisk kraftaktuator og multifunktionel fluorescensbilleddannelse for at studere kernemekanobiologi

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Denne undersøgelse præsenterer en ny protokol til direkte at anvende mekanisk kraft på cellekernen gennem magnetiske mikroperler leveret ind i cytoplasmaet og til at udføre samtidig levende celle fluorescerende billeddannelse.

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Biologi udgave 185 Nucleus mekanobiologi magnetisk kraft CRISPR / Cas9 mekanotransduktion blødt aktivt stof ja-associeret protein (YAP) fluorescerende funktionel billeddannelse digital billedbehandling optogenetik epigenetik
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