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Kombination von 3D-Magnetkraftaktor und multifunktionaler Fluoreszenzbildgebung zur Untersuchung der Kernmechanobiologie
 
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Kombination von 3D-Magnetkraftaktor und multifunktionaler Fluoreszenzbildgebung zur Untersuchung der Kernmechanobiologie

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Diese Studie stellt ein neues Protokoll vor, um mechanische Kraft direkt auf den Zellkern durch magnetische Mikroperlen auszuüben, die in das Zytoplasma abgegeben werden, und simultane Live-Zell-Fluoreszenzbildgebung durchzuführen.

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Biologie Ausgabe 185 Kernmechanobiologie magnetische Kraft CRISPR/Cas9 Mechanotransduktion weiche aktive Materie yes-associated protein (YAP) fluoreszierende funktionelle Bildgebung digitale Bildgebung Optogenetik Epigenetik
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