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JoVE Journal Neuroscience
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
 

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Article DOI: 10.3791/64111-v • 08:26 min • August 23rd, 2022
August 23rd, 2022 •
1, 1, 1, 1, 1
1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo

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Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

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Keywords: Simultaneous Imaging Microglia Dynamics Neuronal Activity Awake Mice AAV Injection Cranial Window Stereotaxic Instrument Motion Artifact Primary Visual Cortex Microinjector
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