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Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
 

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Article DOI: 10.3791/64135-v • 07:03 min • September 23rd, 2022
September 23rd, 2022 •
1,2, 2,3, 2,4, 2,5, 2,5, 2, 2, 2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University

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The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.

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Keywords: Extracellular Vesicles Mesenchymal Stem Cells Isolation Characterization Differential Centrifugation Cell Culture Therapeutic Application PKH26 Dye Particle Tracking Analysis
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