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JoVE Journal Neuroscience
Human Microglia-like Cells
 

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Article DOI: 10.3791/64323-v • 11:19 min • August 18th, 2022
August 18th, 2022 •
1,2, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences, University of Massachusetts Chan Medical School

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This protocol describes the differentiation process of human induced pluripotent stem cells (iPSCs) into microglia-like cells for in vitro experimentation. We also include a detailed procedure for generating human synaptosomes from iPSC-derived lower motor neurons that can be used as a substrate for in vitro phagocytosis assays using live-cell imaging systems.

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Keywords: IPSC-derived Microglia Phagocytosis Assay Human Microglia Microglia Differentiation Primitive Macrophage Precursors Matrigel Coating EB Differentiation
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