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An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
 

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

Article DOI: 10.3791/64562-v • 07:18 min • October 11th, 2022
October 11th, 2022 •
1, 1, 1, 1
1Department of Entomology, University of Minnesota

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Electroporation is a rapid, broadly adopted method for introducing exogenous DNA into the genus Rickettsia. This protocol provides a useful electroporation method for the transformation of obligate intracellular bacteria in the genus Rickettsia.

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Keywords: Rickettsia Electroporation Fluorescent Protein Shuttle Vector Tick Cell Lines Giemsa Staining Cell-free Rickettsia Silicon Carbide Grit Cell-free Preparation Syringe Filter
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