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Biochemistry
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JoVE Journal Biochemistry
Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
 

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Article DOI: 10.3791/64707-v • 09:03 min • March 17th, 2023
March 17th, 2023 •
1, 2, 3, 2, 1, 2, 1
1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific

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Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.

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Mesenchymal Stem Cell Processing Closed Semi-automated Workflow Benchtop Centrifugation Scale-up Challenges Automation Of Dissociation Washing And Harvesting Single-run Protocol Simple Setup Biosafety Cabinet CGMP Facility Reducing Manual Handling Steps Risk Mitigation CGMP Compliance Multi-layered Culture Systems Counterflow Centrifugation System Modification Of Protocol Steps Single-use Counterflow Centrifugation Kit Assembly Tube Welding Wharton's Jelly-derived Human Mesenchymal Stem Cells (HMSCs) Custom Tubing Assembly Sterile Sample Port
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