遺伝学、第124号、合成生物学、CRISPR、Cpf1、C-ブリック、粘着末端、DNAアセンブリ、 Protocols and Video Articles

Research

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Protocols for C-Brick DNA Standard Assembly Using Cpf1

Authors

Author

Institution

¹Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

Journal (Genetics)

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Authors

Author

Amy Smith¹, Mary Bergwell*¹, Ellie Smith*¹, Danita Mathew*¹, Jyoti Iyer¹

Institution

¹Department of Chemistry and Biochemistry, The University of Tulsa

Journal (Genetics)

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Authors

Author

Changyue Liu*^1,2, Zhengyang Lei*^1,2, Lijin Lian², Likun Zhang^1,2, Zhicheng Du^1,2, Peiwu Qin^1,2

Institution

¹Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, ²Institute of Biopharmaceutics and Health Engineering, Tsinghua Shenzhen International Graduate School

Journal (Biology)

| Methods Collections

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Authors

Author

Harriet Prior*¹, Lauren MacConnachie*¹, Jose L. Martinez¹, Georgina C.B. Nicholl¹, Asim A. Beg¹

Institution

¹Department of Pharmacology, University of Michigan

Journal (Genetics)

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Authors

Author

Lifang Yu*¹, Yadan Zhang*¹, Mario Andrea Marchisio¹

Institution

¹School of Pharmaceutical Science and Technology, Tianjin University

Journal (Bioengineering)

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Education

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CRISPR

JoVE Core (Core: Biology)

CRISPR

JoVE Core (Core: Molecular Biology)

CRISPR and crRNAs

JoVE Core (Core: Molecular Biology)

C–C Bond Cleavage: Retro-Aldol Reaction

JoVE Core (Core: Organic Chemistry)

C–C Bond Formation: Aldol Condensation Overview

JoVE Core (Core: Organic Chemistry)

C. elegans Chemotaxis Assay

Science Education (Basic Biology)

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