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CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

DOI:
10.3791/64025-v

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12:14 min

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June 02, 2022

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, ,
1Umeå Centre for Molecular Medicine,Umeå University, 2Department of Histology and Embryology, Medical Academy,Lithuanian University of Health Sciences

Chapters

  • 00:05Introduction
  • 01:00Egg Incubation, Preparation of Weighing Boat, Plastic Boxes, and Dissection Instruments
  • 01:46Opening the Eggs, Transferring to the Internal Humidified Chamber, and Preparing Silicone Rings
  • 03:07Preparation and Seeding the Cancer Cells on the CAM
  • 05:31Isolation of the CAM with Associated Cancer Cells and Sectioning CAM-Delam Samples
  • 07:28Immunohistochemistry Staining, Microscopy Imaging, and Delamination Scoring
  • 09:40Results: CAM-Delam Assay to Evaluate the Metastatic Capacity of Cancer Cells
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

The CAM-Delam assay to evaluate the metastatic capacity of cancer cells is relatively fast, easy, and cheap. The method can be used for unraveling the molecular mechanisms regulating metastasis formation and for drug screening. An optimized assay for analyzing human tumor samples could be a clinical method for personalized cancer treatment.

Tags

CAM-Delam AssayMetastatic PropertiesDelaminationInvasion CapacityCancer CellsMetastatic AggressivenessChick EmbryoEgg IncubationSilicone RingsCollagen RPMI MixCancer Cell CultureTrypsinization

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