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Cancer Research
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CAM-Delam测定通过量化癌细胞的分层和侵袭能力来评分转移特性
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal
Cancer Research
CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
CAM-Delam测定通过量化癌细胞的分层和侵袭能力来评分转移特性
DOI:
10.3791/64025-v
•
12:14 min
•
June 02, 2022
•
Tami Green
,
Lina Šlekienė
2
,
Lena Gunhaga
1
Umeå Centre for Molecular Medicine
,
Umeå University
,
2
Department of Histology and Embryology, Medical Academy
,
Lithuanian University of Health Sciences
Chapters
00:05
Introduction
01:00
Egg Incubation, Preparation of Weighing Boat, Plastic Boxes, and Dissection Instruments
01:46
Opening the Eggs, Transferring to the Internal Humidified Chamber, and Preparing Silicone Rings
03:07
Preparation and Seeding the Cancer Cells on the CAM
05:31
Isolation of the CAM with Associated Cancer Cells and Sectioning CAM-Delam Samples
07:28
Immunohistochemistry Staining, Microscopy Imaging, and Delamination Scoring
09:40
Results: CAM-Delam Assay to Evaluate the Metastatic Capacity of Cancer Cells
11:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
评估癌细胞转移能力的CAM-Delam测定相对快速,简单且便宜。该方法可用于解开调节转移形成的分子机制和药物筛选。用于分析人类肿瘤样本的优化测定可能是个性化癌症治疗的临床方法。
Tags
CAM-Delam Assay
Metastatic Properties
Delamination
Invasion Capacity
Cancer Cells
Metastatic Aggressiveness
Chick Embryo
Egg Incubation
Silicone Rings
Collagen RPMI Mix
Cancer Cell Culture
Trypsinization
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