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CAM-Delam测定通过量化癌细胞的分层和侵袭能力来评分转移特性
JoVE Journal
Cancer Research
Author Produced
This content is Free Access.
JoVE Journal Cancer Research
CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
DOI:

12:14 min

June 02, 2022

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Chapters

  • 00:05Introduction
  • 01:00Egg Incubation, Preparation of Weighing Boat, Plastic Boxes, and Dissection Instruments
  • 01:46Opening the Eggs, Transferring to the Internal Humidified Chamber, and Preparing Silicone Rings
  • 03:07Preparation and Seeding the Cancer Cells on the CAM
  • 05:31Isolation of the CAM with Associated Cancer Cells and Sectioning CAM-Delam Samples
  • 07:28Immunohistochemistry Staining, Microscopy Imaging, and Delamination Scoring
  • 09:40Results: CAM-Delam Assay to Evaluate the Metastatic Capacity of Cancer Cells
  • 11:33Conclusion

Summary

Automatic Translation

评估癌细胞转移能力的CAM-Delam测定相对快速,简单且便宜。该方法可用于解开调节转移形成的分子机制和药物筛选。用于分析人类肿瘤样本的优化测定可能是个性化癌症治疗的临床方法。

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