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Chapters
- 00:00Title
- 01:30Introduction
- 02:10Preparing Reagents
- 03:07Stimulating the Splenocytes
- 05:19Labelling Cells with the Catch Reagent
- 07:36Labeling Cells with IL-17 Detection Antibody (Biotin) and Anti-Biotin-PE
- 09:50FACS Analysis
- 11:40Conclusion
Tags
DetectionIsolationViableMouseIL-17-secreting T CellsMACS Cytokine Secretion AssaySingle Cell LevelSensitive IsolationCytokine-secreting CellsIL-17-secreting CellsSingle Cell SuspensionMouse SplenocytesPMA/ionomycinCytokine SecretionIL-17 Catch ReagentBi-specific AntibodyCD45LeukocytesIL-17Secretion RestartTemperature IncreaseTrapped IL-17Flow CytometryAnti-Biotin-PE Antibody
