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Detektion und Isolation von lebensfähigen Maus IL-17-sezernierenden T-Zellen
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
DOI:

12:38 min

December 18, 2008

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Chapters

  • 00:00Title
  • 01:30Introduction
  • 02:10Preparing Reagents
  • 03:07Stimulating the Splenocytes
  • 05:19Labelling Cells with the Catch Reagent
  • 07:36Labeling Cells with IL-17 Detection Antibody (Biotin) and Anti-Biotin-PE
  • 09:50FACS Analysis
  • 11:40Conclusion

Summary

Automatic Translation

Dieses Verfahren beschreibt die Detektion und Isolation von Maus-TH17 Leukozyten, die sich aktiv sezernieren IL-17 nach Stimulation.

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