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衡量可逆的生物素的血浆膜蛋白吞价格
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Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
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衡量可逆的生物素的血浆膜蛋白吞价格

DOI:
10.3791/1669-v

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11:32 min

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December 23, 2009

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, ,
1University of Massachusetts Medical School

Chapters

  • 00:00Title
  • 00:56Introduction
  • 01:20Cell Culture and Solution Preparation
  • 03:37Biotinylation, Quenching and Internalization of Proteins
  • 07:30Protein Assay, Affinity Chromatography and SDS-Page Analysis of Proteins
  • 09:49Dopamine Transporter Internalization
  • 11:02Conclusion

Summary

Automatic Translation

Automatic Translation

规管的内吞作用管辖的大部分膜蛋白在细胞表面的表达水平。在这里，我们利用还原，膜impermeant生物素化试剂的多巴胺转运蛋白（DAT），一个多面体的膜蛋白的内吞率来衡量的。该方法有利于一个简单的方法来测量大多数等离子的膜蛋白的内吞率。

Tags

Plasma Membrane ProteinsEndocytic RatesReversible BiotinylationProtein TraffickingProtein Surface ExpressionMechanisms Of EndocytosisDirect Endocytic Rate MeasurementsLabeled Receptor LigandsTransportersPumpsIon ChannelsDopamine Transporter (DAT)Internalization RatesTrafficking Studies

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