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从晚期果蝇蛹的大脑原发性神经文化
JoVE Journal
Biology
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JoVE Journal
Biology
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
Please note that all translations are automatically generated.
Click here for the English version.
从晚期果蝇蛹的大脑原发性神经文化
DOI:
10.3791/200-v
•
15:12 min
•
May 28, 2007
•
Beatriz Sicaeros
,
Jorge M. Campusano
,
Diane K. O’Dowd
1
Department of Development and Cell Biology, Department of Anatomy and Neurobiology
,
University of California, Irvine (UCI)
Chapters
00:19
Introduction
01:14
Preparation
01:54
Collection of pupae
02:36
Isolating pupae head
04:13
Isolating brain
05:57
Processing isolated brain
07:04
Washing off enzyme solution
07:58
Trituration and plating
12:23
Imaging calcium flux in pupal neurons
13:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频演示从后期果蝇蛹大脑中的主要神经文化的准备。生活文化的视图显示突起生长的钙水平使用FURA - 2和成像。
Tags
Primary Neuronal Cultures
Late Stage Drosophila Pupae
Brain Removal
Papain Incubation
Serum-free Growth Medium
Mechanical Dissociation
Axons
Dendrites
Regeneration
Live Cultures
GAL4 Lines
Fluorescent Markers
Whole Cell Recordings
Cholinergic Synapses
GABAergic Synapses
Calcium Dynamics
Fura-2 Dye
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