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集落形成细胞（CFC）的含量对人体的造血细胞
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Immunology and Infection
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JoVE Journal Immunology and Infection
Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
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集落形成细胞（CFC）的含量对人体的造血细胞

DOI:
10.3791/2195-v

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11:30 min

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December 18, 2010

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, ,
1Department of Pathology and Immunology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:04Introduction
  • 01:17Thawing and Pre-Activation of CD34+ Cells
  • 03:25Retroviral Transduction by Virus Pre-Loading
  • 05:04Cell Sorting and Collection of Transduced Cells
  • 06:08CFC Assay
  • 08:14Giemsa Staining
  • 10:29CFC Assay Results with Geimsa Stain Analyses
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

集落形成细胞（CFC）的检测是在体外实验中，造血祖细胞形成半固体培养基中的菌落。结合菌落形态，细胞形态，流式细胞仪是用来评估的祖细胞的增殖和分化沿不同的造血谱系能力。

Tags

Colony Forming Cell (CFC) AssayHuman Hematopoietic CellsBone MarrowCord BloodPeripheral BloodHematopoiesisLeukemogenesisLymphoid LineageMyeloid LineageSemisolid MediumProliferationDifferentiation PatternColoniesInput CellsFlow CytometryGiemsa-stained SlidesMyeloid DifferentiationOncogenesPrimary Human CD34+ CellsRetroviral ConstructNUP98-HOXA9MSCV-IRES-GFP

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