Journal
/
Biology
/
从新生小鼠的肺血管内皮细胞的分离和培养
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
Please note that all translations are automatically generated. Click here for the English version.

从新生小鼠的肺血管内皮细胞的分离和培养

DOI:
10.3791/2316-v

•

10:46 min

•

December 14, 2010

•

, ,
1Blood Research Institute,BloodCenter of Wisconsin

Chapters

  • 00:00Title
  • 00:57Introduction
  • 01:58Preparing Anti-PECAM-1 Antibody-Conjugated Magnetic Beads
  • 03:36Isolating Mouse Pulmonary Endothelial Cells from Neonatal Mice
  • 06:56Sorting Mouse Lung Endothelial Cells with Anti-ICAM-2 Dynabeads
  • 09:13Evaluation of Pulmonary Endothelial Cells from Neonatal Mice
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一个小鼠肺血管内皮细胞的分离和培养协议。这种方法包括机械和酶的肺组织分解以及2步纯化过程中使用抗PECAM - 1和抗ICAM - 2的抗体结合磁珠，这将产生一个纯粹的原籍大多微血管的内皮细胞的人口。

Tags

IsolationCulturePulmonary Endothelial CellsNeonatal MiceResearch ModelVascular BiologyHuman Umbilical Vein Endothelial Cells (HUVECs)Microvascular Endothelial Cells (ECs)AngiogenesisPermeabilityKnockout MiceProtein Function AnalysisIsolation ProtocolCulturing ProtocolMouse Lung Endothelial Cells (MLECs)Collagenase/dispase (C/D) SolutionSingle-cell SuspensionPositive SelectionAnti-PECAM-1 AntibodyDynabeadsMagnetic Particle Concentrator (MPC)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2316?article_type=v

瓣膜内皮细胞的分离
check_url/2316?article_type=v

成人从人体皮肤的上皮干细胞的分离和培养
check_url/2316?article_type=v

RNA-seq的凝血酶处理和控制人肺微血管内皮细胞的转录组分析
check_url/2316?article_type=v

分离新生小鼠心肌文化和
check_url/2316?article_type=v

胚胎心室血管内皮细胞的分离
check_url/2316?article_type=v

从鼠标阻力动脉分离微血管内皮管
check_url/2316?article_type=v

肺动脉平滑肌细胞从新生小鼠隔离
check_url/2316?article_type=v

鼠阀门内皮细胞的分离
check_url/2316?article_type=v

隔离和鼠标主动脉内皮细胞原代培养
check_url/2316?article_type=v

人外周血代文化血液的产物内皮细胞

Read Article