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Chapters
- 00:05Title
- 01:06Introduction
- 01:51BiFC Calibration
- 03:04Plating and Transfection of Cells
- 04:37Imaging Cells
- 07:08Monitoring SH3 Interactions of ITSN
- 08:13Conclusion
A localização subcelular de proteínas é importante para determinar a regulação espaço-temporal de sinalização celular. Aqui, descrevemos complementação bimolecular de fluorescência (BiFC) como um método simples para monitorar as interações espaciais de proteínas na célula.
Tags
Bimolecular Fluorescence ComplementationSubcellular DistributionSignaling ComplexesImmunoprecipitationSpatial LocalizationBiFCProtein ComplexesFluororescent ProteinAmino-terminalCarboxy-terminalProtein FusionInteractionReconstitutionFluorophoreLimitationTransient InteractionsWeak InteractionsKinetic AnalysisComplex DynamicsFlourophore MaturationReal Time InteractionsProtein Fragment Complementation AssayReporter ProteinsGreen Fluorescent Protein VariantsDihydrofolate ReductaseB-lactamaseLuciferaseProtein:protein InteractionsFluorescence Co-localizationFörster Resonance Energy Transfer (FRET)
