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Immuno Assay-fluorescentie van Leptospiral Surface-blootgesteld Eiwitten
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1Department of Medicine, David Geffen School of Medicine,University of California, Los Angeles, 2Research service, 151,Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics,University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F,Veterans Affairs Greater Los Angeles Health Care System
Chapters
- 00:05Title
- 02:20Fixation of Leptospira to Glass Slides
- 04:51Labeling with Specific Antibodies
- 06:33Visualization of Leptospires
- 08:42Data Analysis and Representative Results
- 10:20Conclusion
Een efficiënte methode om het oppervlak-blootstelling van leptospiral eiwitten te bepalen is beschreven. De methode is speciaal ontworpen om verstoring van de kwetsbare buitenste membraan van leptospiral cellen te voorkomen. Deze techniek vergt inzet van een aantal negatieve controles te beoordelen van de integriteit van de buitenste membraan en de specificiteit van de antistof reactie.
Tags
Immuno-fluorescence AssayLeptospiral Surface-exposed ProteinsBacterial Surface ProteinsCellular LocalizationFunctional RoleVirulence FactorsPathogenicity MechanismsFractionating Leptospiral MembranesOuter-membrane Proteins (OMPs)LipoproteinsTransmembrane OMPsHydrophobic InteractionLipid Bilayer PhospholipidsAmphipathic β-sheetsBarrel-like StructureOuter Membrane IntegrityImmunofluorescence Assay (IFA)
