Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Targeting Cysteine Thiols for in Vitro…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
 

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

Chapters

Summary
Automatic Translation

Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.

Tags

Keywords: Cysteine Thiols Site-specific Glycosylation Recombinant Proteins Structural Biology Glycosylation Patterns Disease Therapy PCR Mutagenesis DPN1 Digestion Protein Expression
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter