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代谢物的浓度从低密度浮游社区的环境代谢，利用核磁共振光谱

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Biology
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JoVE Journal Biology

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

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代谢物的浓度从低密度浮游社区的环境代谢，利用核磁共振光谱

DOI:

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11:47 min

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April 07, 2012

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R. Craig Everroad*¹, Seiji Yoshida*², Yuuri Tsuboi, Yasuhiro Date, Jun Kikuchi^3,4, Shigeharu Moriya²

¹Biosphere Oriented Biology Research Unit,RIKEN Advanced Science Institute, ²Graduate School of Nanobioscience, Yokohama City University, ³Advanced NMR Metabomics Research Team,RIKEN Plant Science Center, ⁴Graduate School of Bioagricultural Science,Nagoya University

Chapters

00:02Title
01:28Preparation of filters to remove extractables and filtration of sample for metabolite concentration
04:44Extraction of aqueous-soluble metabolites
07:48NMR spectroscopy and data analysis
11:15Conclusions

Summary

Automatic Translation

从微生物浮游社区的代谢产物提取方法。抽样实现整个社会上特意准备的过滤器过滤。冷冻干燥后，溶于水，代谢产物中提取。这种方法用于环境代谢的自然或实验的微生物群落的跨组学研究中的应用。

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