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Journal
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Neuroscience
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E13マウスからグリア制限前駆体の導出
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Derivation of Glial Restricted Precursors from E13 mice
Please note that all translations are automatically generated.
Click here for the English version.
E13マウスからグリア制限前駆体の導出
DOI:
10.3791/3462-v
•
08:56 min
•
June 20, 2012
•
André W. Phillips
2
,
Sina Falahati
2
,
Roshi DeSilva
3
,
Irina Shats
,
Joel Marx
,
Edwin Arauz
,
Douglas A. Kerr
,
Jeffrey D. Rothstein
5
,
Michael V. Johnston
2,6
,
Ali Fatemi
2,6
1
Hugo W. Moser Research Institute at Kennedy Krieger
,
Johns Hopkins University
,
2
Department of Neurology
,
Johns Hopkins School of Medicine
,
3
University of Maryland
,
4
Experimental Neurology
,
Biogen Idec
,
5
The Brain Science Institute
,
Johns Hopkins School of Medicine
,
6
Department of Pediatrics
,
Johns Hopkins School of Medicine
Chapters
00:05
Title
01:05
Tissue Derivation
03:48
Cell Dissociation and Culture Establishment
05:19
Immunopanning the GRP Population
06:27
Freezing and Storage of Derived Cells
07:24
Purified GRP Cells
08:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、胎児の脊髄からグリア制限前駆体の導出の概要を説明し、移植またはオリゴデンドロサイト系譜の研究のいずれかをin vitroで維持されます。
Tags
Glial Restricted Precursor
GRP Cells
White Matter Diseases
Periventricular Leukomalacia
PVL
Oligodendrocyte Progenitor Cells
OPC
Immature Oligodendrocytes
PreOL
Hypoxia-ischemia
Oxidative Stress
Excitotoxicity
Glutamate Excitotoxicity
Oligodendrocyte Progenitors
Transplantation Experiments
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