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赖氨酸去甲基化酶活性的定量测量中的应用的MassSQUIRM
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Biology
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JoVE Journal
Biology
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
Please note that all translations are automatically generated.
Click here for the English version.
赖氨酸去甲基化酶活性的定量测量中的应用的MassSQUIRM
DOI:
10.3791/3604-v
•
07:02 min
•
March 11, 2012
•
Lauren P. Blair
,
Nathan L. Avaritt
,
Alan J. Tackett
1
Department of Biochemistry and Molecular Biology
,
University of Arkansas for Medical Sciences
Chapters
00:05
Title
01:34
LSD1 Demethylation Assay
03:16
Reductive Methylation
04:09
MALDI Mass Spectrometry
04:59
Data Analysis
05:32
MassSQUIRM
06:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个使用MALDI质谱和还原甲基化化学的方法来量化赖氨酸甲基化的变化。
Tags
MassSQUIRM
Quantitative Measurements
Lysine Demethylase Activity
Epigenetic Regulators
Small Molecule Studies
Drug Development
Histones
Proteins
Demethylase Assays
Qualitative Assays
Lysine Methylation States
Mass Spectrometry
Differential Ionization
Comprehensive Analysis
MassSQUIRM Method
Isotopic Reductive Methylation
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