Journal
/
Neuroscience
/
DII-Merking av DRG Neurons til Study aksonal Avgrening i en hel Mount Utarbeidelse av Mouse Embryonic Spinal Cord
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

DII-Merking av DRG Neurons til Study aksonal Avgrening i en hel Mount Utarbeidelse av Mouse Embryonic Spinal Cord

DOI:
10.3791/3667-v

•

09:14 min

•

December 13, 2011

•

,
1Developmental Neurobiology,Max Delbrück Center for Molecular Medicine

Chapters

  • 00:05Title
  • 01:56Dissection Procedure
  • 04:32DiI Labeling of DRG Neurons
  • 06:29Mounting and Microscopic Analysis
  • 07:27Results: Visualizing and Quantification of Single Axonal Trajectories of Embryonic Mouse DRG Neurons
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

Den stereotype projeksjoner av sensoriske afferenter til gnager ryggmargen tilby en lett tilgjengelig eksperimentelt system for å studere aksonal branching gjennom sporing av enkelt axoner.

Tags

DiI-labelingDRG NeuronsAxonal BranchingWhole Mount PreparationMouse Embryonic Spinal CordDiffusive StainingLipophilic Tracer11′-dioctadecyl-333′3′-tetramethylindocarbocyanine Perchlorate (DiI)Axonal PathwaysWild-typeMutated Mouse LinesFunctional RoleCandidate ProteinsControl Of Axonal BranchingWiring Of The Nervous SystemParallel Processing Of InformationIntermediate Target RegionsTerminal ArborizationAxonal GrowthGrowth ConeSplitting BranchingDelayed BranchingInterstitial BranchingCentral Projections Of NeuronsDorsal Root Entry Zone (DREZ)Stereotyped Pattern

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3667?article_type=v

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
check_url/3667?article_type=v

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos
check_url/3667?article_type=v

Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection
check_url/3667?article_type=v

En<em> Ex Vivo</em> Laser-indusert ryggmargsskade Model å vurdere Mekanismer av aksonal degenerasjon i sanntid
check_url/3667?article_type=v

Utarbeidelse av Primary Mixed Glial kulturer fra Adult Mouse Spinal Cord Tissue
check_url/3667?article_type=v

sebrafisk<em> In Situ</em> Spinal Cord Forberedelse til Elektrofysiologiske Opptak fra Spinal sensoriske og motoriske nevroner
check_url/3667?article_type=v

Visualisering av Axonal Projeksjonsmønsteret av Embryoniske Motor Neuroner i<em> Drosophila</em
check_url/3667?article_type=v

Laminectomy og vindus kabel i mus
check_url/3667?article_type=v

Utarbeidelse av akutt ryggmargen skiver for hele celle Patch-klemme opptak i Substantia Gelatinosa nerveceller
check_url/3667?article_type=v

Intraarteriell levering av nevrale stamceller til rotte- og mushjernen: Søknad om cerebral iskemi

Read Article