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切向流超：A胶体银纳米粒子的大小选择与集中“绿色”方法
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JoVE Journal Biology
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
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切向流超：A胶体银纳米粒子的大小选择与集中“绿色”方法

DOI:
10.3791/4167-v

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12:47 min

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October 04, 2012

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, , , , , , ,
1Department of Chemistry,Wright State University, 2Department of Neuroscience, Cell Biology, and Physiology,Wright State University

Chapters

  • 00:05Title
  • 02:23Synthesis of Colloidal AgNPs
  • 04:07UV-Vis Spectrophotometry: Surface Plasmon Resonance
  • 05:17Tangential Flow Ultrafiltration: Size-selection and Concentration
  • 07:17Inductively Coupled Plasma Optical Emission Spectroscopy: Quantification of Silver
  • 08:37Size Distribution of Colloidal AgNPs via Transmission Electron Microscopy (TEM)
  • 09:22Results: Synthesis and Characterization of Colloidal AgNPs
  • 11:43Conclusion

Summary

Automatic Translation

Automatic Translation

切向流超滤（TFU），是一个循环的生物样品的重量为基础的分离方法。 TFU适于大小选择（1-20 nm直径）和高度集中大量的多分散性的银纳米颗粒（4 L的15.2微克毫升<sup> -1</sup8,539.9微克毫升下降到4毫升<sup> -1</sup>），以最小的聚集。

Tags

Tangential Flow UltrafiltrationColloidal Silver NanoparticlesAgNPsSize SelectionConcentrationNanoparticle ApplicationsAntimicrobial PropertiesControlled FabricationSize-based IsolationChemically Aggressive Capping/stabilizing AgentsOrganic SolventsToxicity Of ReagentsHigh CostsReduced EfficiencyAgNP SynthesisIsolation MethodsCentrifugationSize-dependent SolubilitySize-exclusion ChromatographyTFU (Tangential Flow Ultrafiltration)Creighton AgNPsUltracentrifugationRecirculation MethodHollow Fiber MembranesPore Size

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