Journal
/
/
评估肾细胞​​蛋白激酶C同工酶,线粒体功能及细胞活力的
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
DOI:

15:43 min

January 07, 2013

,

Chapters

  • 00:05Title
  • 02:27Primary Cultures of Renal Proximal Tubular Cells
  • 05:24Measurement of RPTC Respiration
  • 06:18Analysis of Mitochondrial Membrane Potential (ΔΨm)
  • 07:14Isolation of Mitochondria
  • 08:16Assaying Enzyme Complexes of the Respiratory Chain
  • 10:24Measurement of Intracellular ATP Content
  • 10:55Analysis of Cell Viability
  • 12:18Results: Overexpressing PKC-ε in RPTCs
  • 14:56Conclusion

Summary

Automatic Translation

活化的蛋白激酶C(PKC)的同工酶与呼吸,氧化磷酸化有关的线粒体功能和对细胞活力的影响进行说明。该方法适应腺病毒技术,选择性过度表达PKC同工酶在原代培养和各种检测,以确定线粒体功能和能量状态的细胞。

Related Videos

Read Article