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评估肾细胞蛋白激酶C同工酶,线粒体功能及细胞活力的
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Biology
Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Please note that all translations are automatically generated.
Click here for the English version.
评估肾细胞蛋白激酶C同工酶,线粒体功能及细胞活力的
DOI:
10.3791/4301-v
•
15:43 min
•
January 07, 2013
•
Grażyna Nowak
,
Diana Bakajsova
1
Department of Pharmaceutical Sciences, College of Pharmacy
,
University of Arkansas for Medical Sciences
Chapters
00:05
Title
02:27
Primary Cultures of Renal Proximal Tubular Cells
05:24
Measurement of RPTC Respiration
06:18
Analysis of Mitochondrial Membrane Potential (ΔΨm)
07:14
Isolation of Mitochondria
08:16
Assaying Enzyme Complexes of the Respiratory Chain
10:24
Measurement of Intracellular ATP Content
10:55
Analysis of Cell Viability
12:18
Results: Overexpressing PKC-ε in RPTCs
14:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
活化的蛋白激酶C(PKC)的同工酶与呼吸,氧化磷酸化有关的线粒体功能和对细胞活力的影响进行说明。该方法适应腺病毒技术,选择性过度表达PKC同工酶在原代培养和各种检测,以确定线粒体功能和能量状态的细胞。
Tags
Protein Kinase C
PKC Isozymes
Mitochondrial Function
Cellular Energy Status
Cardioprotection
Nephrotoxicant-induced Mitochondrial Dysfunction
Cell Death
Renal Cells
Oxidative Phosphorylation
Cell Survival
Renal Proximal Tubular Cells (RPTC)
Adenoviral Vectors
PKC-epsilon Mutant CDNAs
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