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皮肤纹身作为一种新的方法DNA疫苗交货
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Bioengineering
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JoVE Journal Bioengineering
Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
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皮肤纹身作为一种新的方法DNA疫苗交货

DOI:
10.3791/50032-v

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06:37 min

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October 18, 2012

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, , , ,
1Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 2Department of Pathology,New York University School of Medicine, 3Healthcare System,Veterans Affairs New York Harbor

Chapters

  • 00:00Title
  • 00:15Introduction
  • 01:22Plasmid DNA Preparation
  • 02:10Tattoo Equipment Setup
  • 03:26Animal Skin Preparation
  • 04:14DNA Tattooing
  • 05:33Visualization of EGFP Expression
  • 06:04Conclusion

Summary

Automatic Translation

Automatic Translation

皮肤纹身是一个强大的和安全的方式来传递DNA疫苗皮内。在这里，一个DNA质粒EGFP交付纹身实验室小鼠的皮肤，和EGFP在皮肤细胞中的表达，然后通过激光共聚焦显微镜检查。

Tags

Keywords: DNA VaccineNucleic Acid-based VaccinationPlasmid DNASkin TattooingIntradermal DeliveryEGFPConfocal MicroscopyAdjuvantCost-effectiveConvenient

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