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脑幽静的白细胞的分离与分析<em>伯氏疟原虫</emANKA感染的小鼠
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
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脑幽静的白细胞的分离与分析<em>伯氏疟原虫</emANKA感染的小鼠

DOI:
10.3791/50112-v

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12:48 min

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January 02, 2013

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, ,
1The Walter and Eliza Hall Institute of Medical Research

Chapters

  • 00:05Title
  • 01:52Infection of Mice with P. berghei-ANKA
  • 06:49Isolation of Brain-sequestered Leukocytes (BSL)
  • 09:10Immunophenotyping of BSL by Multicolor Flow Cytometry
  • 10:27Representative Analysis of Isolated BSL Populations
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

贴壁脑血管的炎性白细胞隔离的方法<em>伯氏疟原虫</em> ANKA感染的小鼠进行说明。可以量化的方法，以及后的孤立白细胞表型特征流式细胞仪荧光抗体染色和随后的分析。

Tags

Keywords: Brain-sequestered LeukocytesPlasmodium Berghei ANKAExperimental Cerebral MalariaIntracardial PerfusionPercoll GradientFlow CytometryInflammatory LeukocytesParasitic Infection

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