Journal
/
Bioengineering
/
在激光诱导轴突病变张力释放测量评估轴突在Piconewton和毫秒级的分辨率对基材的附着力
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
Please note that all translations are automatically generated. Click here for the English version.

在激光诱导轴突病变张力释放测量评估轴突在Piconewton和毫秒级的分辨率对基材的附着力

DOI:
10.3791/50477-v

•

09:31 min

•

May 27, 2013

•

, ,
1Institute of Biophysics,National Research Council of Italy, 2Dipartimento di Sistemi e Informatica,Università di Firenze, 3Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia

Chapters

  • 00:05Title
  • 01:27Laser Microsphere Manipulation
  • 03:31UV and IR Beam Positioning and Optical Tweezer Calibration
  • 04:56Axotomy and Simultaneous Force Measurement
  • 06:50Results: Measurement of Changes in Cytoskeletal Equilibrium Caused by Laser Induced Lesions
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

我们测量了张力释放部分病变进行附着膜的轴突的光学捕获探针上的的同时力显微镜测量用激光剥离器通过在一个轴突。所开发的实验协议评估轴突的培养基材的粘附性。

Tags

Laser-induced Axon LesionTension ReleaseAxonal AdhesionForce SpectroscopyInterferometric TrackingOptical TrappingCell-substrate CouplingNeurite GrowthNeuronal Regeneration

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

循环压力为生物反应器的设计体外主动脉心脏瓣膜的研究

构建一个低预算的激光干切断系统研究轴突再生 C。线虫</em

基质贴壁细胞的微管吸吮的估计单元刚度

使用Cell-基板阻抗和活细胞成像，以衡量矩阵修改诱导实时变化的细胞粘附和去粘附

通过电场诱导释放和测量（EFIRM）检测外体生物标志物

导管内送货到兔乳腺

预测神经植入物软化行为的环境动力力学分析

集成张力传感器在亚微米分辨率下的成像整合和细胞力

人类诱导多能干细胞衍生心肌细胞中的单细胞光学作用潜在测量

DNA张力探针用于绘制免疫细胞的瞬时皮牛顿受体力

Read Article