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隔离的感觉神经元中<em>海兔丫</em谷氨酸能电流膜片钳记录

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

Please note that all translations are automatically generated. Click here for the English version.

隔离的感觉神经元中<em>海兔丫</em谷氨酸能电流膜片钳记录

DOI:

10.3791/50543-v

•

09:40 min

•

July 10, 2013

•

Lynne A. Fieber, Stephen L. Carlson, Andrew T. Kempsell, Justin B. Greer, Michael C. Schmale

¹Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Sciences,University of Miami

Chapters

00:05Title
01:44Day One: Cell Preparation
04:12Day Two: Neuron Isolation
08:24Patch Clamp Recordings of Glutamatergic Currents in the Sensory Neurons of Aplysia
08:49Conclusion

Summary

Automatic Translation

我们描述了解剖海洋海兔的神经系统<em>海兔</em麻醉后短期组织培养的神经元，和单细胞离子电流通过膜片钳技术记录的隔离。

Tags

Aplysia Californica Sensory Neurons Patch Clamp Glutamatergic Currents Marine Gastropod Mollusk Nervous System Function Learning And Memory Neuronal Co-culture Dissociated Neurons Ion Currents Biophysical Characterization Pleural Ganglia Buccal Ganglia

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